IL3RA 抗体 (PE)
Quick Overview for IL3RA 抗体 (PE) (ABIN488142)
抗原
See all IL3RA 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- This antibody reacts with Human CD123 antigen (IL-3 receptor alpha-chain).
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交叉反应 （详细）
- Species reactivity (tested):Human.
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纯化方法
- Protein-A Sepharose Chromatography.
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免疫原
- COS cells overexpressing IL-3 receptor alpha-chain. Remarks: Hybridoma was established by fusion of mouse myeloma cell NS-1 with Balb/c mouse splenocyte
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亚型
- IgG1
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anti-Interleukin 3 Receptor, alpha (IL3RA) (AA 200-305) antibody
IL3RA 适用: 人 FACS, IHC, ELISA, ICC 宿主: 小鼠 Monoclonal 8E11C5 unconjugated
anti-Interleukin 3 Receptor, alpha (IL3RA) antibody (PE)IL3RA 适用: 人 FACS 宿主: 小鼠 Monoclonal 6H6 PE
anti-Interleukin 3 Receptor, alpha (IL3RA) (AA 19-158) antibodyIL3RA 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 3 Receptor, alpha (IL3RA) (AA 201-300) antibodyIL3RA 适用: 人, 小鼠 FACS, WB, ELISA, IHC (p), IHC (fro), IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 3 Receptor, alpha (IL3RA) (AA 167-331) antibodyIL3RA 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 3 Receptor, alpha (IL3RA) (AA 1-100) antibodyIL3RA 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 3 Receptor, alpha (IL3RA) antibodyIL3RA 适用: 人, 小鼠, 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 3 Receptor, alpha (IL3RA) (AA 26-171) antibodyIL3RA 适用: 人 IHC, ELISA, Coat, ISt, StM 宿主: 兔 Monoclonal IL3RA-2947R unconjugated Recombinant Antibody
anti-Interleukin 3 Receptor, alpha (IL3RA) antibodyIL3RA 适用: 人 FACS, WB, IP, IHC (fro) 宿主: 小鼠 Monoclonal 6H6 unconjugated
anti-Interleukin 3 Receptor, alpha (IL3RA) (AA 19-305) antibodyIL3RA 适用: 人 FACS, ELISA 宿主: 兔 Monoclonal DM31 unconjugated
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应用备注
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Flow Cytometry: 20 μL (Ready for Use). Positive Control: Transfectant cells. Detailed procedure is provided in the following Protocols.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
实验流程
- Flow Cytometric Aanalysis for Adherent CellsProtocol 1We usually use Fisher tubes or equivalents as reaction tubes for all steps after 2). 1) Detach the cells from culture dish by trypsinization. *Excessive trypsinization mayreduce the antigenicity. 2) Wash the cells 3 times with washing buffer [PBS containing 2% fetal calf serum (FCS)and 0. 1% NaN3]. 3) Resuspend the cells with washing buffer (5x10e6 cells/mL). 4) Add 50 µL of the cell suspension into each tube, and centrifuge at 500 x g for 1 minute atroom temperature (20~25°C). Remove supernatant by careful aspiration. 5) Add 10 µL of normal goat serum containing 1 mg/mL normal human IgG and 0. 1% NaN3to the cell pellet after tapping. Mix well and incubate for 5 minutes at room temperature(20~25°C)6) Add 20 µL of the PE labeled CD123 monoclonal antibody (6H6). Mix well, and incubatefor 30 minutes at room temperature. 7) Add 1 mL of the washing buffer followed by centrifugation at 500 x g for 1 minute at roomtemperature (20~25°C). Remove supernatant by careful aspiration. 8) Resuspend the cells with 500 µL of the washing buffer and analyze by a flow cytometer. Positive Control for Flow Cytometry: transfectant
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- PBS containing 1 % BSA as stabilizer and 0.09 % Sodium Azide as preservative.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Store the antibody undiluted at 2-8 °C.
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- IL3RA (Interleukin 3 Receptor, alpha (IL3RA))
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别名
- CD123 / IL3RA
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背景
- CD123 is the IL-3-specific component of the IL-3 receptor. CD123 binds IL-3 only in combination with CDw131, the Synonyms: IL-3R-alpha, IL3 receptor alpha, Interleukin-3 receptor subunit alpha
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基因ID
- 3563
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NCBI登录号
- NP_002174
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UniProt
- P26951
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途径
- JAK/STAT Signaling
抗原
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